大枣果肉用乙醇回流脱脂,80～100℃热水浸提,提取液经减压浓缩,乙醇分级沉淀,三氯乙酸脱蛋白,透析,透析内液再经减压浓缩、醇沉得到三个粗多糖ZJ-A’,ZJ-B’,ZJ-C’,ZJ-B’经DEAE-Cellulose柱、Sephacryl S-300柱反复纯化,得大枣多糖ZJ-1、ZJ-2,高效凝胶渗透色谱(HPGPC)和常压凝胶柱色谱分析表明,ZJ-1、ZJ-2为均一多糖,HPGPC法测定分子量(MW)分别为9848、8991,紫外吸收光谱表明,ZJ-1、ZJ-2无核酸和蛋白质的特征吸收峰。

The flesh of Zizyphus jujube MILL is defatted by the way of ethanol reflux and extracted in 80-1000C water; the extracted liquid is concentrated by reducing its pressure, and the fractions ZJ-A, ZJ-B and ZJ-C are obtained by fractional precipitation of the liquid with ethanol and clearing away protein from it by CC13COOH. As the result, ZJ-land ZJ-2 are obtained via further purification of ZJ-B and their DEAE -Cellulose and Sephacryl S-300 column chromatography, and their homogeneity is verified by HPGPC and Sephacry S-300. No absorption peaks of protein and nucleic acid are found from Zj-1 and Zj-2 by UV-scan and the molecular weights of Zj-1 and Zj-2 are 9848 and 8991 respectively by the determination of HPGPC.

河南重大科技攻关项目(984021200)