中药复方配伍理论是中药方剂的精华。中药配伍后多化学成分的变化研究尚未见报道。本文首次应用HPLC-DAD研究川芎-赤芍配伍的多化学成分变化。由于中药成分复杂,需要建立稳定、重复、可靠的液相色谱分析方法;然后用液相色谱的统一方法分离样品和数据处理;通过比较合煎液和单煎液中各成分的保留时间和紫外光谱确定合煎液中各峰的归属;并可研究配伍后化学成分含量的变化。实验结果表明,川芎-赤芍配伍后少数化学成分含量发生变化,但没有发现新峰的产生。

The theory of compound compatibility is the essence in the prescriptions of traditional Chinese medicine （TCM）. No research work has been reported on the change of multi - components in TCM compatibility up to now. In this article the study on the change of multi - components in the compatibility of Radices paeoniae rubra and Ligusticam wallichii by means of HPLC - DAD is first delt with. Owing to the complex components of Chinese medicines, it is necessary to have a stable duplicated and reliable analysis of high - performance liquid chromatography - photodiode array detector （HPLC - DAD） to separate samples and process data in the study. By comparing retention time and UV spectra of all the components in the liquid of co - decoction and individual decoction of Radices paeoniae rubra and Ligusticam wallichii, the peak of each component in the liquid of the co - decoction can be identified and the change of chemical components in the compatibility of Radices paeoniae rubra and Ligusticam wallichii can also be clarified. The research result indicates that the content of a few chemical components has changed, but no new component has emered after compatibility.

国家科学技术部973资助项目(G19990554)