目的:通过对补血药白芍主要效应成分芍药苷在血虚证和健康小鼠体内血药浓度比较,探讨血虚证与补血药的辨证药物动力学关系。方法:建立血虚证动物模型,将实验动物分为空白对照组,对照组（健康小鼠）,实验组（血虚证动物模型）,对照组和实验组均给予白芍提取物灌胃。采用高效液相色谱技术,比较两组小鼠灌胃后不同时间点血浆中芍药苷浓度的差异。结果:实验组小鼠平均血药浓度在60 min、90 min时明显高于对照组,实验组小鼠血浆中芍药苷平均血药浓度为116．52±5．28 ng/μl,而对照组为41．49±2．86 ng/μl,二者比较有显著差异（p<0．001）。结论:血虚证小鼠血浆中芍药苷浓度较健康小鼠高,说明血虚证与补血药白芍存在辨证药物动力学关系。

Objective: To explore the relationship of dialectic pharmaco-dymamics between the disease due to the deficiency of blood and blood-tonifying drugs via the comparison of blood concentration of paeoniflroin, the main component of Radix Paeoniae Alba for blood - tonifying drugs, in the body of mice which are healthy and suffer from the disease of blood deficiency respectively. Method: To establish models of animals suffering from the disease due to blood deficiency and divide experimental animals into blank control group, control group （healthy mice） and experimental group （model of animals contracting the disease due to blood deficiency）, among which mice of control group and experimental group are forced to fill the extracts from Radix Paeoniae Alba into their stomach and then the technology of high performance liquid chromatography is used to test and compare the difference of concentration of paeoniflroin in the blood of the mice at different time. Result: The average concentration of paeoniflroin in the blood of the mice of experimental group is 116. 52±5. 28ng/μl while that of the mice of control group is 41. 49±2. 86mg/μl, assuming remarkable difference batween the two groups （p <0.001） and that of the former is notably higher than that of the latter when at 60 min and 90 min. Conclusion: the concentration of paeoniflroin in the blood of the mice suffering from the disease due to blood deficiency proves higher than that of healthy mice and this shows that there exists the relationship of dialectic pharmaco -dynamics between the disease due to blood deficiency and blood - tonifying drug Radix Paeaniae Alba.

国家中医药管理局科研基金(02-03ZP50):活血温里复方中温里药提高活血药生物利用度的研究，负责人：杨祖贻；四川省中医管理局科研基金(200217):加味四物复方中温里药对活血药增效机理研究，负责人：杨祖贻。