视网膜神经节细胞（retinal ganglion cells,RGCS）凋亡是青光眼视神经损害的最终共同通路。而钙拮抗药能通过多种环节阻断视网膜神经节细胞凋亡,起到视神经保护作用。由此我们提出: 钙拮抗作用与视神经保护可能存在一定的相关性。以灯盏细辛有效组分为代表,采用45Ca同位素技术对其钙拮抗有效组分进行筛选,同时结合体外常压、高压视网膜神经细胞培养实验,考察其钙拮抗作用与视神经保护之间是否存在一定的相关性。本文综合了几方面的实验结果,通过初步相关性分析,表明灯盏细辛有效组分钙拮抗作用与视神经保护之间存在较好的相关性。

The apoptosis of retinal ganglion cells and the damage of optic nerves in glaucoma have their common passage, but antagonist drugs with calcium are able to block up the apoptosis of retinal ganglion cells by many links, thus playing a role of protecting optic nerves. Therefore, the authors of this article suggest that there must be a given correlationship between calcium antagonism and the protection of optic nerves. In their studies the authors chose Erigeron breviscapus as an example to screen its effective components by the technology of 45Ca isotope and took in vitro constant and high -pressure experiment of retinal ganglion cell culture to investigate whether there exists the correlationship between the calcium antagonism of this herb and the protection of optic nerves. By summarizing the results of the experiment from several aspects and preliminarily analyzing their correlationship it is demonstrated that there is a best correlationship between the calcium antagonism of the effective components of Erigiron breviscapus and the protection of optic nerves.

国家自然科学基金项目(30271581): 应用(45)Ca同位素对视神经保护中药有效成分的筛选方法研究,负责人：张艺。