目的:研究治疗痛风加味四妙丸的药效物质基础,确定有效部位在各药效靶点上的作用,追踪有效部位（群）。方法:分离纯化加味四妙丸中的各类成分即生物碱、皂苷、黄酮、有机酸、挥发油及水液部分,以二甲苯所致小鼠耳肿胀抗炎实验、醋酸引起小鼠化学性刺激镇痛实验及高尿酸模型下的降尿酸活性为指标,筛选确定抗炎、镇痛有效部位;并将各有效部位进行正交组合筛选,确定该处方的有效部位群及在各药效靶点上的作用。结果:这六个部位组合后的抗炎、镇痛和降尿酸活性与处方药一致,是该处方的有效部位群;各部位组合配伍后,挥发油在抗炎、镇痛、降尿酸活性上均起主要作用。

To study the material foundation of the effectiveness of Jiaweisimiaowan, a Chinese patent drug for arthrolithiasis, determine the functions of its effectiveness and trace its components. Method: To separate and purify the components of the said patent drug, e. i., alkalord, saponin, flavore, organic acid, volatile oil and water and screen and determine its effective components for anti - inflammation and analgesia by taking for indexes the results of the anti -inflammatory experiment of mice's ears with dimethylbenzene, the analgesia experiment by stimulating mice with acetic acid and the experiment of reducing the activity of uric acid in a hyperuric model, and identify the group of effective components of the drug and its functions on the targets of effectiveness through perpendicularly combined screening of all the effective components. Result: The functions of anti - inflammation, analgesia and the reduction of the activity of uric acid of the 6 combined components are in accord with those of the said patent drug and thus the 6 ones make up the group of effective components of it. Volatile oil plays the major role in anti - inflammation, analgesia and the reduction of the activity of uric acid through the combination and compatibility of all the components.

江苏省自然科学基金项目(BK2002033)治疗痛风加味四妙丸的筛选及有效部位群的研究,负责人：尹莲。