目的:温胆汤改良方含药脑脊液及含药血清对AD细胞模型凋亡及凋亡相关蛋白bcl- 2、bax表达的影响。方法:温胆汤改良方含药脑脊液和含药血清分别作用于Aβ25-35诱导NG108-15 细胞建立的AD细胞模型,镜下观察细胞生长状况,流式细胞术检测细胞凋亡,免疫组化检测bcl- 2、bax蛋白的表达。结果:与模型组比较,含药脑脊液组和含药血清组细胞凋亡率降低（p<0．05）, bel-2表达无显著性差异（p>0．05）,bax蛋白表达减弱（p<0．01）,bcl-2／bax比值升高（p<0．01）。结论:温胆汤改良方能有效地抑制AD细胞模型凋亡,其作用机理可能与其干预凋亡相关基因bax的表达,升高bcl-2／bax比值有关。

Objective To explore the effect of decoction-containing cerebrospinal fluid and serum of improved Wendan prescription on the apoptosis and the expression of protein bcl-2 and bax associated with apoptosis in cellular model of Alzheimer's disease. Methods To respectively apply decoction-containing cerebrospinal fluid and decoction-containing serum to cellular model of Alzheimer's disease established by Aβ25-35 inducing NG108-15 cells and then observe the growth of cells under microscope, testing the apoptosis of cells by flow cytometric analysis and the expression of the protein bcl-2 and bax by the method of immunohistochemistry. Results In comparison with the model group the rate of cell apoptosis is lower （p<0.05）, the expression of bcl-2 assumes no apparent difference （p>0.05）, the expression of bax protein becomes weaker （p<0.1） and the ratio of bcl-2/bax goes up （p<0.1） in the groups of decoction-containing cerebrospinal fluid and decoction-containing serum. Conclusion The improved prescription of Wenden decoction is able to effectively inhibit the apoptosis of cellular model of Alzheimer's disease and its functional mechanisms may be associated with its intervention in the expression of the gene bax related to apoptosis and the rise of bcl-2/bax ratio.

国家中医药管理局基金项目(NO．04-05JP41)：温胆汤改良方对Alzhcimer病细胞模型神经细胞周期影响的实验研究，负责人：王平。