目的:采用单侧输尿管结扎（UUO）肾间质纤维化模型,探讨复方鳖甲软肝方对肾间质纤维化大鼠血生化及肾组织病理形态学的影响。方法:将大鼠随机分为7组,正常组、假手术组、模型组、苯那普利组和复方鳖甲软肝方低、中、高剂量组。UUO术后4周处死大鼠,并应用HE、六胺银染色及Mallory染色,观察肾组织病理学改变。结果:模型组肾小管结构严重破坏,管腔塌陷,上皮细胞大量坏死;有些部位肾小管和间质的结构基本消失,间质中出现弥漫性细胞增殖,并广泛间质纤维化。复片鳖甲软肝方组和苯那普利组纤维化病理改变明显减轻,其中以复片鳖甲软肝方中剂量组作用明显。结论:复方鳖甲软肝方可减少炎细胞的浸润、减轻肾小管的损伤,进而延缓肾间质纤维化的病程进展。

Objective To investigate the action of the compound prescription Biejiarangan on the blood biochemistry and the pathomorphology of renal tissues of rats with interrenal fibrosis by the way of an unilateral ureteral obstruction （UUO） model. Method To divide the rats for experiments into the following seven groups:normal, sham operation, model, Benazepril treatment and three groups of rats fed with low, medium and high dosages of the compound prescription Biejiarangan respectively ,and to kill the rats in four weeks after UUO operation and to dye with HE,PAM and Mallory in order to observe the pathological change of the renal tissues of the rats. Result In the model group the structure of all the kidney tubules of the rats is heavily damaged with their lumina callapsed and their epithelial cells are largely in necrosis. In some parts the structure of the kidney tubules and the mesenthyme of the rats basically disappear and cell proliferation emerges in the mesenthyme in a diffuse way and the fibrosis of the mesenthyme gets widespread. In the groups of the rats fed with low, medium and high dosages of the compound prescription Biejiarangan and in the group of Benazepril treatment the pathological change of interrenal fibrosis of the rats becomes apparently mitigated and it is particularly evident in the group of the rats fed with medium dosages. Conclusion The compound prescription Biejiarangan is able to reduce the infiltration of inflammatory cells, lessen the damage of kidney tubules and then retard the progress of interrenal fibrosis.

国家自然科学基金重点项目(30130220)：复方鳖甲软肝片防治多脏器纤维化的作用机理研究，负责人：牛建昭。