目的:为川白芷药材质量研究提供新的评价方法和依据。方法: 对样品进行HPLC测定, 采用主成分分析、聚类分析及相似度评价等多种方法进行评价,并对川白芷规范化种植中不同试验项目样品进行了指纹图谱的比较。结果:熏硫和未熏硫样品的指纹图谱存在较大差异,分别建立了熏硫和未熏硫川白芷药材的指纹图谱。结论:本文所建立的指纹图谱较全面地反映了川白芷香豆素类成分的特征,可作为川白芷质量评价的方法,并可用于区分川白芷是否熏硫。

Objective To provide the study of the quality of the medicinal plant Radix angelicae anomalae with new method and basis of assessment. Method To test the samples of Radix angelicae anamales by HPLC and assess them by such methods as the analysis of main components, cluster analysis and similarity assessment and to compare via fingerprint the samples used in different experiments in the cultivation of Radix angelicae anomalae. Result There exist significant differences between the finger-printing of sulphured samples and that of unsulphured ones and as the result the finger-prints of sulphured and unsulphured samples of this medicinal plant are established respectively. Conclusion The finger-print mentioned in this article epitomizes the characteristics of coumarin family of Radix angelicae anomalae and therefore this method can be used for the assessment of the quality of the medicinal plant and also for the distinction of whether the products of this plant are sulphured or not.

科学技术部863计划项目(2001BA701A40-3)：白芷配方颗粒质量标准的规范化研究，负责人：马逾英。