目的:探讨复方鳖甲软肝方对LPS诱导的人近端肾小管上皮细胞增殖的影响。方法:由 UUO大鼠制备含药血清作为药源加入离体反应系统,MTT比色法检测各组血清对LPS诱导的肾小管上皮细胞增殖的影响。结果:于24h、48h及60h,阳性药物组及复方鳖甲软肝方各组OD值均低于模型组,而阳性药物组OD值与复方鳖甲软肝方各组相比无显著差异。结论:复方鳖甲软肝方可抑制 LPS诱导的肾小管上皮细胞增殖,从而延缓肾间质纤维化进程。

Objective To study the effect of the compound prescription Biejiarangan on the proliferation of the LPS-induced cells of near-end kidney tubules. Method To prepare drug-contained serum from rats with UUO to enter into in vitro reaction system and then detect the effect of all serum sections on the proliferation of LPS-induced epithelial cells of Kidney tubules by the MTT colorimetric method. Result In 24 hours, 48 hours and 60 hours the OD value of the rats in the model group is higher than that in the normal group and the OD value of the rats fed with positive drugs and all the groups of rats fed with the compound prescription Biejiarangan is lower than that of rats in the model group while there is little difference between the OD value of group rats fed with positive drugs and that of all groups of rats fed with the compound prescription Biejiarangan. Conclusion The compound prescription Biejiarangan is able to inhibit the proliferation of LPS-induced epithelial cells of Kidney tubules, thus delaying the progress of interenal fibrosis.

国家自然科学基金重点项目(30130220)复方鳖甲软肝方防治多脏器纤维化作用机理研究教育部“长江学者和创新网队 发展计划”项目(IRT0413)中医药干预多器官纤维化与异病同治、同病异治科学内涵的研究