目的:用反相高效液相色谱法（RP-HPLC）对地参70％（V／V）乙醇提取物进行分析方法的研究。方法:考察了不同波长、酸抑制剂浓度、流动相梯度以及进样量对其分离效果的影响。结果:确定了分析方法:Hypersil ODS2色谱柱（5μm,4．6mm i．d．×250mm）;流动相乙腈-水-甲酸（0．5％,V／V）;洗脱梯度0-5min（10％ B）,5-70min（10％-60％ B）;流速1．0 mL／min;检测波长280nm;进样量3μL;柱温308。结论:该方法获得的色谱图分离度较高,基线较平稳,能有效地对地参进行化学成分分析与质量控制。

This article presents the study on the method for analyzing 70% （v/v） ethanolic extract of Rhezoma lycopi by means of reversephase high performance liquid chromatography （RP-HPLC） and the impact of different wavelengths, the concentration of inhibitives, the gradients of mobile phase and the injection volumes on the effect of the separation of the components of the said medicinal plant. In the study the following methods have been used: Hypersil ODS2 chromatographic column （5μm, 4.6mm, i.d.×250mm）, mobile phase acetomitrile-water-formic acid （0.5%,v%）, 0-5min （10%B） and 5-70 min （10%-60%B） of elution gradient, 1.0mL/min of flow velocity,detection wavelength 280nm, injection volume 3μL and the temperature of chromatographie column 30℃. The chromatogram obtained by the said method appears with rather high separative degree and stable base line so that the analysis of chemical components and the quality control of Rhezoma lycopi can be effectively conducted on by it.

中国科学院知识创新工程重要方向项目(KGCX2-SW-213)基于现代理论和技术的复方中药系统研究国家自然科学基金重 大项目(3053886)中国重要药用植物类群的亲缘学的研究