目的:对苗药苦针汤进行组方基础研究和新药开发研究。方法:以该方对金黄色葡萄球菌、痢疾杆菌、白色葡萄球菌、致病性大肠杆菌（γ）的抑制作用为考察指标,运用混料均匀设计法将组成苦针汤中的4味药配制成12种不同比例的复方,通过抗菌实验,测试用药抗菌能力。结果:建立回归方程,对回归方程及其中各项系数进行检验,求抗菌效果最佳时的变量取值。结论:回归方程通过检验,有意义、可用;苦参、黄芩对抑菌效果的贡献不显著,其余两味药以19．56g和11．84g组方时抗菌效果最佳,优于理论计算值。

Objective To study the composition of the prescription of Kuzhentang decoction, a traditional medicine of Miao nationality in China, in order to lay a foundation for the development of new drugs. Method To take the inhibition of this prescription on Staphylococcus aureus, Shigella dysenteriae, Staphylococcus albus and Pathogenic colibacillus （Y） as the index of investigation and choose four medicinal herbs from the prescription of Kuzentang decoction to prepare 12 compound prescriptions by the method of uniform design of blending materials so as to test their ability of antibiosis and establish a regression equation, and then detect the regression equation and all its parameters in order to achieve the variable value of optimal, antibiotic effect. Conclusion The regression equation is of significance and applicable. The two medicinal herbes radix Sophora flavescens Ait. and Scutellaria baicalensis Georgi of the prescription do not have remarkable effect on Pathogenic colibacillus, but the other two composed of 19.56g and 11.84g respectively assume optimal effect on antibiosis, whose value is better than that from theoretical computation.

贵州省中药现代化科技生产研究开发专项重大课题资助项目(黔科合中药专字[2003]07)新药“长安灵”研制