为鉴别中药材黄芪的品种及其代用品,采用高盐低pH值法提取药材的基因组DNA,利用随机扩增多态DNA(RAPD)技术扩增药材基因组DNA样品。结果表明,高盐低pH值法较适用于黄芪类药材基因组DNA的提取,其中2个引物可作为高特异性引物,根据琼脂糖凝胶上显示的DNA带型差异准确鉴别黄芪和红芪。RAPD方法可以准确、快速地鉴定黄芪及其代用品。

To identify Chinese traditional medicine (TCM)“Huangqi”(Astragalus membranaceus) and its substituents,the total genomic DNA of fourteen samples was obtained using high saline-low pH value method,and was amplified by randomly amplified polymorphic DNA (RAPD).The result indicated that the method of high saline-low pH value is propitious to extract high-quality total genomic DNA from Astragalus membranaceus.The samples of Astragalus membranaceus and Hedysarum polybotrys could be distinguished by two primers according to the banding patterns of their amplified DNA on agarose gel after electrophoresis.Therefore,RAPD is able to identify Huangqi and its sub- stitutes quickly and correctly.