[关键词]
[摘要]
目的:观察龙葵总碱对S180小鼠及H22小鼠肿瘤细胞膜Na+,K+-ATPase及Ca++,Mg++- ATPase活性的影响。方法:荷瘤小鼠分为治疗组（高剂量、中剂量、低剂量）和对照组（生理盐水组、环磷酰胺组）,分别测定各组肿瘤细胞膜的Na+,K+-ATPase及Ca++,Mg++-ATPase活性。结果:龙葵总碱对S180小鼠及H22小鼠肿瘤细胞膜Na+,K+-ATPase及Ca++,Mg++-ATPase活性均有明显的抑制作用,并且其抑制作用呈量效正相关。结论:龙葵总碱这一作用可能是其抗肿瘤作用机理之一。
[Key word]
[Abstract]
Objective:To observe the effect of total alkaloids （TA） from Solarium nigrum on the activities of Na+,K+-ATPase and Ca++,Mg++-ATPase in tumor cells'membrance of S180 and H22 mice. Methods: Mice were divided into treated group （high, medium and low dosage） and control group （normal saline and cyclophosphamide）, and the activities of Na+,K+-ATPase and Ca++,Mg++-ATPase in tumor cells'membrance were examined measured respectively. Results:TA showed pronounced inhibition on the activities of Na+,K+-ATPase and Ca++,Mg++-ATPase at in tumor cells'membrance, and it showedand the inhibition showed dose-effect positive relationship. Conclusion: This effect may be one of the mechanisms of its antitumor effect.
[中图分类号]
[基金项目]
国家自然科学基金项目(30400591):中药龙葵中生物碱促进转染阴离子通道(AEI)膜段域K562细胞凋亡作用及机制研究;;黑龙江省自然科学基金资助项目(D2004-13):龙葵总碱促进转染带3蛋白跨膜域K562细胞凋亡作用机制的研究
