目的:探讨绞股蓝总皂甙（Gp）对人肝细胞瘤细胞（Huh-7）细胞凋亡的影响及作用机制。方法:采用流式细胞术检测Gp对人Huh-7细胞凋亡的影响,Western blot分析Gp作用Huh-7细胞后表达的Bcl-2,Bcl-XL,Bax和Bad蛋白水平。结果:20mg/ml Gp作用于细胞24h后,64％的Huh-7细胞发生凋亡,而人纤维细胞凋亡率为12％。Westem blot结果显示Gp作用后Huh-7细胞中Bcl-2表达明显降低,而Bax表达显著上调。结论:Gp可诱导人Huh-7细胞凋亡,其作用机制是通过下调Bcl-2和上调Bax的表达而发挥作用。

Objective:To study the proapoptotic effects of Gp on human hepatoma cells. Methods:The effects of Gp on the cell apoptosis of human hepatoma cell line Huh-7 was assessed by flow cytometrial analysis, and the expression of Bcl-2, Bel-XL, Bax and Bad molecules in Huh-7 after treated by Gp was detected by using Western blot analysis. Results:After treatment with 20mg/ml Gp for 24 h, 64% of Huh-7 cell were undergoing apoptosis, while cell death was only observed in 12% of human synovial fibroblast cells treated by Gp. Western blot analysis demonstrated that, Bcl-2, an anti-apoptotic molecule, was markedly decreased in Huh-7 cells treated by Gp. Whereas, proapoptotic molecule Bax was significantly up-regulated in Huh-7 cells after Gp treatment. Conclusion: Our results suggest that treatment of human hepatoma cells with Gp induced apoptosis through the down -regulation of Bcl -2, and up -regulation of Bax.