目的:研究芦丁、槲皮素、山萘酚等黄酮类成分在毛细管中的迁移行为与电压、缓冲溶液pH值及其离子浓度的关系。方法:在室温下,分别考察电压、缓冲液pH值及缓冲液离子浓度对分离度的影响。结果:3种黄酮类物质在pH值8．65、电压20KV、缓冲液离子浓度12mmoL／L条件下为最佳。结论:对于3种物质在毛细管电泳中的迁移行为,缓冲液的pH值对各峰的分离度起主要作用,电压和缓冲液的离子浓度主要影响各峰的出峰时间.

Objective: To study the migration of Rutin, Quercetin and Kaemperol in HPCE. Methods: The vdtage of HPCE.pH and concentration of buffer were changed respectively to study the effect for separation. Results: The shape peaks and separation were proved to be good under the condition which pH was 8.65,and voltage was 20kv and concentration was 12mmol/L. Conclusion: The separation degree of the three compounds was mainly determined by pH of buffer, and migration time of them were mainly determined voltage and concentration of buffer.

黑龙江中医药大学校基金项目(200519):高效毛细管电泳法评价洋金花、类叶牡丹质量的研究