目的：建立用RP-HPLC技术同时测定黄连中小檗碱、巴马亭、黄连碱及药根碱等4种原小檗碱型生物碱含量的方法。方法：采用高效液相色谱法。测定黄连药材中上述4种原小檗型生物碱的含量。结果：4种原小檗碱型生物碱的平均含量分别为：5．74%（RSD=1．7%）（n=9）,1．33%（RSD=1．3%）（n=9）,3．67%（RSD=2．8%）（n=9）,0．95%（RSD=5．2%）（n=9）。结论：该方法可用于同时测定黄连中4种原小檗碱型生物碱的含量,且简便、准确、可靠、快速,并具重现性。

This study is to establish a quantitative method for analyzing the concentration of four berberine-type alkaloids in Coptis chinensis Franch in a simultaneous manner,using RP-HPLC.The RP-HPLC process,made up of Hypersil- 18 Column （250mm×4.6mm,5μm）,a mixed mobile phase of acetonitrile-Water（48:52,KH2PO4 3.4g,SDS 1.7g every IL）,a flow velocity of 1.0 mL·min-1,and a detection wavelength of 345nm,is employed to simultaneously de- termine the concentration of four Berberine-type Alkaloids in Coprtis chinensis Franch.The study has produced the fol- lowing results:linear calibration curves for Berberine,Palmaune,Coptisine,and Jatrorrhizine in a respective range of 0. 024～0.096mg·mL-1 and r=0.9994;0.01155～0.03005mg·mL-1 and r=0.9994;0.00298～0.02679mg·mL-1 and r=0.9992;and 0.0032～0.0096mg·mL-1 and r=0.9992.The average recovery rates are registered respectively at 102.3% and RSD=3.0%（n=3）;101.5% and RSD=1.8%（n=3）;103.7% and RSD=3.6% （n=3）;and 113.3% and RSD=7.0% （n=3）.The average concentration of four berberine-type alkaloids in Coptis chinensis Franch is measured at5.74%（RSD=1.7%）（n=9）;1.33%（RSD=1.3%）（n=9）;3.67%（RSD=2.8%）（n= 9）;and 0.95% （RSD=5.2%） （n=9） respectively.It is concluded that the method is simple,accurate,reliable, quick and reproducible.It can be used for quantitative analysis of four berberine-type alkaloids in Coptis chinensis Franch in a simultaneous manner.

山东省自然科学基金项目(Y2002C07)：中药全息指纹图谱智能质量控制方法学研究