目的：研究内吸性化学杀虫剂吡虫啉对金银花中绿原酸含量的影响。方法：采用高效液相色谱法,色谱柱为十八烷基键合硅胶柱,流动相为乙腈-0．4%磷酸（13：87）；检测波长为327nm。结果：吡虫啉对金银花中绿原酸的含量有一定的影响,施药后绿原酸含量与对照相比有所升高,而且随施用药剂浓度增加,绿原酸含量增加。结论：吡虫啉对金银花绿厚酸的含量有影响,其中20%吡虫啉可溶性液剂4000倍液对金银花中的绿原酸含量有显著影响。

Objective:determining the possible effects on the concentration of chlorogenic acid in Flos lonicerae,after the ap- plication of imidacloprid （20% SL）.Methods:Chlorogenic acid is separated using Spherisorb C18 column,and Aceto- nitrile （0.4% phosphatic acid at 13:87）,with a measured wavelength at 327nm.Results:Imidacloprid is of a seen effect on the concentration of chlorogenic acid,which is raised after the application of imidacloprid.Conclusion:Imida- cloprid has an effect on the concentration of chlorogenic acid.An Imidacloprid of 20% SL with a 4000-time dilution produces noticeable effects on the concentration of chlorogenic acid in Flos lonicerae.

国家中医药管理局项目(02-03ZP03)：农药在中药材害虫防治安全性研究