对灌胃给予大鼠降香总黄酮后,进入体内的化学成分和Ⅱ相代谢产物进行了初步研究。采用酶水解的方法将Ⅱ相代谢产物水解为原型形式,以HPLC-DAD和HPLC-MS技术进行样品分析,采用对照品对照的方法,在水解前、后的生物样品中分别定性分析了18个黄酮类成分。结果表明大鼠灌胃降香总黄酮后,黄酮类成分在体内发生了Ⅱ相代谢反应,药物在体内同时存在原型和Ⅱ相代谢产物两种形式,Ⅱ相代谢产物又以硫酸酯结合物为主。

In this paper,flavonoids in free form and phaseⅡreactive metabolites in rats,after oral administration of D.odorif- era extract,are investigated.The sulfate and glucuronide conjugates of the flavonoids in rat are studied by hydrolysis withβ-glucuronidase/sulfatase utilizing HPLC-DAD and HPLC-MS techniques.By comparison with the authentic standards,eighteen flavonoids in free form are found in rat serum and urine,both before and after the enzyme treatment. It shows that the phaseⅡreaction has taken place in rats,after oral administration of D.odorifera total flavornoids.Fla- vonoids are present in rats in both free and conjugated forms.Further studies show that sulfation is the primary conjuga- tion in rat,while glucuronidation is not noticeable.

科学技术部“创新药物与中药现代化”重大专项(2001BAC01A56)：70种中药注射剂指纹图谱研究及其技术平台的建立;;国家中医药管理局专项基金(2004ZX01)：中药指纹图谱关键技术与对照谱库建立的方法学研究;;科学技术部科技基础性工作专项(2002DEA20021)：食品、中药与天然药物有效成分检测技术研究