目的：考察大鼠肠道茵群对淫羊藿苷和淫羊藿次苷Ⅱ的代谢情况。为研究淫羊藿苷的口服吸收机理打下基础。方法：将待测药物与新配制的大鼠肠内容物混悬液在37℃孵化,采用高效液相色谱法同时测定孵化前后溶液中淫羊藿苷和淫羊藿次苷Ⅱ的浓度。结果：淫羊藿苷被肠道菌群迅速代谢成淫羊藿次苷Ⅱ,而淫羊藿次苷Ⅱ不被代谢。结论：研究淫羊藿次苷Ⅱ的吸收和代谢机理对于研究淫羊藿苷的口服吸收机理非常有必要。

The purpose of this study is to understand the metabolism of icariin and its main metabolite icarisideⅡby rat intes- tinal bacteria in vitro,creating a basis for further study of oral icariin absorption.In the study,Icariin and icarisideⅡwere incubated together with rat intestinal bacteria at a temperature of 37℃.An HPLC method was used for simultane- ous determination of Icariin and icarisideⅡ.It is found that Icariin is rapidly metabolized to icarisideⅡ,though icarisideⅡis not touched.The finding suggest that it is important to understand the metabolism and absorption mechanism of ica- risideⅡ,in an attempt to better understand the oral absorption mechanism of icarisideⅡ.

国家科技部973项目(2007CB507400)：衰老的机制与干预的基础研究