DNA条形码（DNA barcoding）是根据对一段标准的DNA序列的分析来鉴定物种,已成为生物物种鉴定的新方向,受到世界40多个国家130多个组织中传统生物分类学家、分子生物学家和生物信息学家等多学科专家的关注。本文通过介绍DNA条形码的产生、发展和研究现状,探讨其在中药材鉴定中应用的技术方法、技术路线、关键问题以及应用范围,并展望了DNA条形码在中药材鉴定中的应用前景。

To identify biological species, DNA barcoding as a new technique is developed. DNA barcoding sequences from a standardized and agreed-upon position in the genome are very short relative to the entire genome and they can be obtained reasonably quickly and cheaply, which attracted more than 130 organizations from 40 countries in the globe. This paper reviews the appearance and development of DNA barcoding. Simultaneously, we study the methods, stepwise technique map and key questions of DNA barcoding which was used to identify Chinese medicinal materials. In conclusion, DNA barcoding has a good prospect in authentication of Chinese medicinal materials.

国家自然科学基金项目(30572324):濒危品种川贝母光合蒸腾特性与野生抚育生态因子的相关性研究,负责人:陈士林。