采用衍生化GC/MS的方法分析尿液中内源性小分子代谢物, 结合主成分分析、最小二乘法等模式识别方法计算峰响应信号,冷应激前后正常大鼠尿液内源性代谢物在主成分分析图上产生显著分离,人参皂苷组则冷应激前后几乎无分离。利用单维统计对差异物质加以检验,发现其中与应激相关的代谢物如酪氨酸、色氨酸等物质的相对含量产生显著变化,据此推测机体对冷刺激的应答以及应激后自我调节的过程,而这些物质中绝大多数在人参皂苷组中变化的显著性降低或者已无显著性。本文从代谢物的角度证实冷应激对动物机体的影响、机体的自我恢复以及人参皂苷的抗应激作用。

Acute stress may trigger systemic biochemical and physiological changes in living organisms, leading to a rapid loss of homeostasis, which can be gradually reinstated by self-regulatory mechanisms and/ or drug intervention strategy. Urinary metabolite profiling of Sprague-Dawley rats exposed to cold temperature （-10℃） for 2 h using derivatized GC/MS in conjunction with modern multivariate statistical techniques revealed drastic biochemical changes as evidenced by fluctuations of urinary metabolites and demonstrated the protective effect of ginsenosides on stressed rats. The metabonomics approach enables us to visualize significant alterations in metabolite expression patterns as a result of stress-induced metabolic responses and post-stress compensation. The protective actions of ginsenosides were verified by reduced variations of endogenous metabolites caused by cold stress.

国家自然科学基金项目(30572286):基于代谢组学的肾阳虚证本质的研究,负责人:蒋健。