目的:建立大黄素体内含量测定方法,对虎杖主要药效成分大黄素在大鼠体内的药代动力学及在肝脏分布的经时变化进行研究,以反映虎杖药材在大鼠体内的作用规律.方法:采用HPLC方法检测生物样品中的大黄素含量,绘制血药浓度-时间曲线、肝脏药物浓度-时间曲线.结果:大黄素在大鼠体内药代动力学模型符合二房室模型,血药浓度给药后9 min达到峰值,实测的Cmax=3.973±0.535 μg/mL.半衰期约为11小时,肝脏药物浓度时间曲线显示,肝脏中药物浓度给药后10 min达到峰值,实测的Cmax=9.654±1.41 μg/g.给药24小时,血浆及肝脏中药物浓度均显著降低.结论:虎杖中大黄素在大鼠体内吸收入血迅速,并快速分布至肝脏.该研究可能对与大黄素结构相似的蒽醌类成分体内代谢的研究具有参考价值,有助于阐明虎杖药理作用的物质基础.

This paper discusses the relationships between liver controlling dredging and both Estrogen and Progesterone receptors' expression. Authors observe both Estrogen and Progesterone receptors' expression variations across different CNS areas (lower part of Thalamus, Border leaf), using molecular biological technique. They found that when liver controlling dredging leads to a Liver- qi invasion, gene expressions of both Estrogen and Progesterone receptors would be reduced in a noticeable manner. Progesterone receptor has a reduced gene expression mainly in the lower part of Thalamus, while Estrogen receptor mainly in the Border leaf. It is concluded that the reduced gene expression of Progesterone and Estrogen receptors can be used as an indicator for diagnosing Liver- qi Invasion.

科技部科技基础工作专项( 2007FY130100) : 道地中药材及主要成分的标准物质研制与分析方法研究, 负责人: 马辰。