[关键词]
[摘要]
目的：鉴定六味地黄丸中的化学成分和代谢成分。方法：采用超快液相色谱梯度洗脱，飞行时间质谱正负离子同时检测。结果：在六味地黄提取物中鉴定了40 种化学成分，在大鼠血中鉴定了6 种原型药物和9 种代谢产物，并推断了以上产物的代谢途径。结论：UFLC-ESI-IT-TOF 方法适合于复杂中成药样品的分析，有助于全面了解六味地黄丸中的化学成分和代谢成分。
[Key word]
[Abstract]
Objective: To identify the chemical and metabolic components. Method: UFLC gradient elution with TOF detection in positive and negative mode. Result: 40 chemical components were identified in Liuwei Dihuang extract. 6 original component and 9 metabolites were identified in rat serum. The proposed metabolic pathway was given. Conclusion: UFLC-ESI-IT-TOF was a powerful method in the analysis of complicated TCM samples. It helps the comprehensive understanding of the chemical and metabolic components of Liuwei Dihuang Pills.
[中图分类号]
[基金项目]
国家科技支撑计划（2006038079037）：基于代谢组学的人参皂苷Rh2、四物汤等研究，负责人：许长江；国际科技合作项目（2007DFA31060）：代谢组学用于中药质量控制的方法学研究，负责人：许长江.
