目的：采用反相高效液相色谱法（RP-HPLC）测定了芍药苷对血管紧张素转化酶活性的影响。方法：采用Brava BDS C18色谱柱（4.6mm×250mm，5μm），流动相为乙腈-0.01%甲酸水溶液（25：75），柱温为30 ℃，流速为1.0 mL·min-1 ，检测坡长为228nm。结果：马尿酸在1.17~149μg·mL-1（r = 0.9999）范围内呈良好的线性关系；平均回收率（n=6）为100.4%（RSD=0.88%）；日内、日间精密度的RSD分别为1.32%和1.96%。芍药苷的IC50值为37.47μmol·L-1。结论：该法分离效果和重复性均良好，结果准确可靠，可用于马尿酸的检测，也为血管紧张素转化酶抑制剂活性测定提供了可靠的方法。

This is a study attempting to develop an efficient RP-HPLC method for determining the inhibitory activity of angiotensin converting enzyme (ACE). The content of enzymolysis product hippuric acid was determined using RP -HPLC method. RP-HPLC was performed on a Brava BDS C18 column (250 mm×4.6 mm, 5 um) using a mobile phase consisting of 0.01％ formic acid solution-acetonitrile (25:75) at 30 ℃ with a flow rate of 1.0 mL·min - 1 and a detection wavelength at 228 nm. Test results showed that the calibration curve was linear in the range of 1.17-149 μg·mL-1 (r = 0.9999) for hippuric acid. The average recovery (n=6) of hippuric acid was 100.4% (RSD=0.88%). The intra-day and inter-day RSDs were at 1.32% and 1.96% respectively. The IC50 of Paeoniflorin was 37.47 μmol·L-1. Enjoying a sensitive, accurate, and reliable performance with good reproducibility, the method is desirable for determining the inhibitory activity of ACE．

北京市重点实验室（JD100260652）项目：清热解毒中药有效成分发现研究，负责人：乔延江。