利用高速逆流色谱技术，一次进柱即可实现从附子粗提物中分离得到苯甲酰新乌头原碱。两相溶剂系统用氯仿-甲醇-0.3mol/L盐酸三元系统，上相为固定相，下相为流动相，转速为892rpm，流速为1.2ml/min。通过ESI-MS、13C-NMR、1H-NMR鉴定，确认了其化学结构。经HPLC分析，纯度达98%以上。

Authors separated Benzoylmesaconitine from raw aconite in an immediate manner using HSCCC. A methanol-methylene chloride-hydrochloric acid (-0.3mol/L) solvents system was used, with a rotation speed at 892rpm and a flow rate 1.2ml/min, making the upper part the fixed phase, and the lower part the mobile phase. The chemical composition was determined through ESI-MS, 13C-NMR, and 1H-NMR screening. HPLC test showed a purity as high as 98% or above.

国家科技部科技支撑计划课题（2008BAI49B05）：戒毒中药济泰片的药效物质、作用机制与质量标准研究，负责人：沈平孃。