目的：建立大黄黄连泻心汤浸渍剂中主要化学成分同时测定的HPLC方法，并分析在配伍黄芩前后这些主要化学成分的变化。方法：采用HPLC-UV法，色谱柱为苯基柱（250mm×4.6mm， 5μm），柱温为室温，流动相为乙腈-0.5%三乙胺（磷酸调节pH至3.5），梯度洗脱，流速为1mL/min；紫外检测波长为254nm、270nm，进样量为20?滋L。结果：本研究建立的HPLC方法准确、可靠，可同时测定大黄黄连泻心汤的主要化学成分；在大黄和黄连配伍黄芩前后浸渍剂中的主要化学成分发生了明显变化，大黄蒽醌类成分明显增加，黄连生物碱中小檗碱含量明显减少。结论：大黄黄连泻心汤的组方中是否配伍有黄芩将对该方主要化学成分含量变化产生明显影响。

A HPLC method was developed and validated to quantify 9 major marker chemicals of Dahuang Huanglian Xiexin Decoction (DHXD) after soaking different combinations of the constituent herbs in boiling water for various time periods. The HPLC was equipped with a phenyl column (250mm×4.6mm, 5μm) and a UV detector set at 254 nm and 270 nm. Separation of DHXD chemical markers was achieved using acetonitrile-0.5% triethylamine with pH adjusted to 3.5 by phosphoric acid and gradient elution. The HPLC method was accurate and reliable in quantifying the concentrations of the DHXD chemical markers . In the present study, the effects of Scutellaria baicalensis on the marker chemical concentrations were examined closely. When S. baicalensis was included in the DHXD formulation, anthraquinones concentrations were found to increase significantly, but berberine concentration was diminished. Result of the study shows that S. baicalensis is able to significantly change the concentrations of the DHXD markers.

国家自然科学基金项目（30873428）：大黄黄连泻心汤主要化学成分分析与生理药动学研究，负责人：姚美村。