利用电喷雾串联质谱方法（ESI-MS/MSn）和内标法分析麻黄附子甘草汤方剂中二萜类生物碱种类和含量的变化，通过拆方研究了麻黄、甘草对附子生物碱的影响规律，揭示了麻黄附子甘草汤的配伍内涵。研究表明，麻黄、甘草单独与炮附子配伍时能够通过化学和物理作用减少热解型单酯型生物碱的含量，并生成脂型生物碱，但方剂组合后有效成分热解型单酯型生物碱的含量增加。结合初步药效学实验表明，热解型单酯型生物碱是具有明显的抗炎作用，说明炮附子在复方中发挥抗炎作用的主要成分可能为热解型单酯型生物碱，同时复方的整体配伍利于有效成分的转化。

Norditerpenoid alkaloids in Mahuang-Fuzi-Gancao-Tang was studied using Electrospray Ionization Tandem Mass Spectrometry and internal standard method. By measuring the structures and relative amount of norditerpenoid alkaloids in Mahuang-Fuzi-Gancao-Tang, the effects of Mahuang or Gancao on norditerpenoid alkaloids in the processed Fuzi was revealed. Mahuang and Gancao could reduce the abundance of pyrolytic monoester-diterpenoid alkaloids (P-MDAs) in the processed Fuzi, and increase the amount of lipo-alkaloids through chemical reactions and physical absorption. Meanwhile, the amount of P-MDAs has registered an apparent increase in the whole prescription. Pharmacological experiments also revealed that P-MDAs is a major anti-inflammatory component in the processed Fuzi, and the compatibility of the prescription is desirable for the effective transformation of constituents.

国家自然科学基金项目（30472134）：麻黄附子甘草汤”化学物质基础及其质谱方法学研究，负责人：宋凤瑞；国家自然科学基金项目（30672600）：中药复杂体系药效物质基础的分析方法学研究，负责人：刘淑莹；吉林省科技发展计划项目（20080105）：中药附子中具有强心活性的低毒成分筛选及结构鉴定项目资助，负责人：皮子凤。