目的：建立HPLC法测定西藏不同产地的铁棒锤、榜嘎中双酯型生物碱的含量方法。方法：色谱柱：AichromTM C18（4.6mm×150mm，5?滋m)；柱温：35℃；流动相：甲醇-0.05mol·L-1磷酸二氢钾-醋酸-异丙醇（67:173:4:4）；检测波长：230nm；流速：1.0mL·min-1。结果：乌头碱在0.09632～0.4816?滋g（r=0.9998）范围内呈线性。乌头碱加样回收率为96.3%，RSD为1.87%（n=6）。结论：方法可控，结果稳定，为乌头类药材含量测定提供一个参考方法。

This study aimed to establish a determination method of diester-alkaloids in Aconitum szechenyianum and Aconitum naviculare. The chromatographic method was carried out on the AichromTMC18 column using CH3OH-0.05mol·L-1 KH2PO4-CH3COOH-（CH3）2CHOH (67:173:4:4) as a mobile phase. The detection wavelength was set at 230nm， with the flow rate of 1.0 mL·min-1 and the column temperature of 35℃. The aconitine calibration curves were found to be linear in the range of 0.09632~0.4816?滋g（r=0.9998）. The average recovery of aconitine was 96.3% with RSD=1.87%. This method is simple and reliable, providing a reference standard for the quality control of aconitum alkaloids.

西藏自治区科技厅支持项目：15种藏药材质量标准研究，负责人：易进海。