目的：建立高效液相色谱法（HPLC）测定排毒清脂片中的大黄素、大黄酚的含量。方法：色谱柱：UItimate XB－C18柱（250mm×4.6mm，5?滋m）；流动相：甲醇－磷酸溶液（85∶15）；流速：1mL·min-1；检测波长：254nm。结果：大黄素、大黄酚分离良好，进样量分别在0.007984~0.047904?滋g和0.01002~0.06012?滋g范围内与峰面积是良好线性关系，r值分别为0.9991和0.9998，平均回收率分别为99.18%和99.94%，RSD分别为1.19%和1.16%（n=5）。结论：该方法简便、精确、分离效果好、专属性强，可作为排毒清脂片中大黄成分的质量控制方法。

HPLC was established for the determination of the rhubarb content of poison discharging and lipid clearing tablets. Using XB-C18 column (250mm × 4.6mm, 5?滋m) as the chromatographic column and methanol-phosphoric acid (85∶15) as the mobile phase, emodin and chrysophanol were well separated at the flow rate of 1mL·min-1 and wavelength of 254nm. Their volumes showed a good linear relationship with the area respectively in the range of 0.007984~0.047904?滋g and 0.01002~0.06012?滋g. The r value was 0.9991 and 0.9998, the average recovery 99.18% and 99.94%, and RSD 1.19% and 1.16% (n = 5) respectively for emodin and chrysophanol. The results showed that this method is simple and accurate, with high separating efficiency and good specificity. All these features make HPLC a candidate measure for the quality control of the rhubarb content of poison discharging and lipid clearing tablets.