目的:探索黄连体外改善胰岛素抵抗活性与HPLC指纹图谱的相关性.方法:采用RP-HPLC法建立不同产地的黄连样品的指纹图谱,采用主成分分析法进行化学模式识别研究,并采用体外测定药物改善脂肪细胞胰岛素抵抗生物活性,对化学信息和生物效应进行灰色关联分析和多元相关分析,以探索两者的相关性.结果:不同产地黄连药材化学成分含量及生物效应的有一定的差异.HPLC指纹图谱各共有峰中色谱峰10、6、3、7和巴马汀与改善胰岛素抵抗活性相关性较强.结论:基本明确了化学指纹图谱与改善胰岛素抵抗生物效应的相关性,有利于黄连改善胰岛素抵抗活性成分的筛选.

The fingerprints of Rhizoma coptidis from various producing areas were established by High performance liquid chromatography (HPLC), and the activity of R. coptidis in ameliorating insulin resistance in vitro was studied by colorimetry. The HPLC fingerprints were evaluated using the similarity analysis (SA) and the principal component analysis (PCA). The correlation between HPLC fingerprints and activity of of R. coptidis in ameliorating insulin resistance in vitro was investigated using the grey correlation analysis (GCA) and the multiple correlation analysis (MCA). Peak 10, peak 6, peak 3, peak 7 and palmatine in the HPLC fingerprints were found to be the main active constituents to ameliorate insulin resistance. Some ecological factors of the producing areas, such as the climate, the latitude and longitude, artificial factors might also influence the function of R. coptidis. This work primarily revealed the correlation between HPLC and amelioration of insulin resistance by R. coptidis, which may help to screen the active ingredients of R. coptidis to ameliorate insulin resistance.

科技部国家"十一五"科技支撑计划地方专项课题(2007BAI40B05):体现道地性与中医药特色相关的川贝母、白芷、黄连质量标准系统研究，负责人：张艺；科技部国家"十一五"科技支撑计划地方专项课题(2007BAI40B03):川产道地药材川贝母、白芷、黄连的系统研究与开发，负责人：范昕建。