目的:采用HPLC梯度洗脱法同时测定决明子中橙黄决明素、大黄素、大黄酚和大黄素甲醚的含量.方法:用Kromasil 100A C18色谱柱(5μm,4.6mm×150mm,迪马公司);甲醇-0.1%磷酸溶液梯度洗脱:0～15min(65:35),15～16 min(65～85:35～15),16～40 min(85:15);柱温:室温;检测波长:285nm(0～15min),254nm(15～40min);流速:1mL·min-1.结果:橙黄决明素、大黄素、大黄酚和大黄素甲醚的线性范围分别为0.00038～0.00608μg(r=0.9998)、0.0029～0.0464μg(r=0.9999)、0.00105～0.0168μg(r=0.9996)、0.00044～0.00704μg(r=0.9998),平均回收率分别为99.50%(RSD=0.71%)、99.59%(RSD=1.42%)、99.31%(RSD=0.59%)、99.74%(RSD=0.91%).结论:本方法专属性强,重复性好,可用于决明子中橙黄决明素、大黄素、大黄酚和大黄素甲醚的含量测定.

This study aimed to simultaneously measure the content of aurantio-obtusin, emodin, chrysophano1 and physcione and in semen cassiae by HPLC. A Kromasil 100A C18 column (5μm, 4.6mm×150mm, DIKMA) was used for the gradient elution with a methanol-0.1％ phosphonic acid solution: 0~15min (65:35)，15~16min (65~85: 35~15)，16~40min (85:15), at the flow rate of 1 mL·min-1 at room temperature．The UV wavelength was detected at 285 nm (0~15min) and 254nm (15~40min). The linear range was determined to be 0.00038~0.00608μg (r=0.9998), 0.0029~0.0464μg (r=0.9999）, 0.00105~0.0168μg (r=0.9996) and 0.00044~0.00704μg (r=0.9998） respectively for aurantio-obtusin, emodin, chrysophano1 and physcione, with the average recovery of 99.50% (RSD=0.71%）, 99.59% (RSD=1.42%）, 99.31% (RSD=0.59%) and 99.74% (RSD=0.91%）. The results showed that this method has good specificity and repeatability, and thus considered as a good tool for the measurement of aurantio-obtusin, emodin, chrysophano1 and physcione in semen cassiae.

科技部国家自然科技资源共享平台项目(2005DKA21000):药用植物种质资源标准化整理、整合及共享试点--子课题:"川牛膝、决明、小决明、穿心莲、大麻、赤小豆等，负责人：卫莹芳（总课题负责人：黄璐琦）。