目的：探讨金银花体外抗结核活性。方法：使用试管倍比稀释法测定金银花、绿原酸、异烟肼对结核分支杆菌标准株和金色分支杆菌的最小抑菌浓度（Minimum Inhibitory Concentration，MIC）及最小杀菌浓度（Minimum Bactericidal Concentration，MBC）。结果：金银花对金色分支杆菌的MIC为0.83mg?mL-1，MBC为1.75mg?mL-1，对H37Rv的MIC、MBC均为0.18mg?mL-1；绿原酸对两种分支杆菌的MIC、MBC均为0.18mg?mL-1；异烟肼对两种分支杆菌的MIC、MBC均为10.5?滋g?mL-1，异烟肼配伍金银花使用，异烟肼对两种分支杆菌的MIC、MBC为5.25?滋g?mL-1。结论：金银花有抗结核活性。同异烟肼联合使用能显著降低异烟肼的MIC和MBC。

This work aimed to explore the bioactivities in vitro of Flos lonicerae against Mycobacterium tuberculosis (TB ). Minimum inhibitory concentration and minimum baictericidal concentration of Flos lonicerae, cholorogenic acid and INH against M. tuberculosis and M. aurum were determined by the serial tube dilution method. The MIC of Flos lonicerae to Mycobacterium aurum was determined to be 0.83mg?mL-1, and the corresponding MBC was 1.75mg?mL-1; for cholorogenic acid, the MIC and MBC were both 0.18mg?mL-1; for INH, the MIC and MBC were both 10.5?滋g?mL-1. When INH and Flos lonicerae were used in combination, INH had the MIC and MBC of 5.25?滋g?mL-1. The results showed that Flos lonicerae exhibited antituberculosis activities in vitro, and the combined usage of INH and Flos lonicerae could significantly reduce the MIC and MBC.