目的:研究酶法结合传统回流法提取远志总皂苷工艺条件.方法:利用可见分光光度法测定远志总皂苷含量;考察植物复合酶的酶解时间、酶解温度对传统提取工艺的强化效果;通过显微结构图和红外光谱图说明了复合酶法强化传统提取法的作用方式及提取物的结构.结果:酶用量为0.4%时,适宜酶解温度为55℃,最优酶解时间为0.5h.结论:复合酶法结合传统回流提取后,可提高远志总皂苷提取率、缩短提取时间、减少乙醇使用量,对于远志流浸膏的高效提取具有一定指导意义.

This work investigated the extraction of total saponin in Polygala using the enzymolysis combined with the traditional reflux. The content of total saponin in Radix Polygala was determined by a visible spectrometer, and the strengthed effect of enzymolysis time and enzymolysis temperature was studied on the tradition extraction process. The function of the combined enzymolysis-strengthened traditional extraction and the structure of the extracts were determined by the infrared absorption spectrum and microstructure spectrum. The best quantity of enzyme was found to be 0.4%，the enzymolysis temperature 55℃，and the optimal enzymatic treatment 2.5h. The results showed that this method can increase the extraction rate of total saponin in Polygalae, shorten the extraction time, and reduce the consumption of ethanol, thus helping the extraction of polygala liquidum.

山西省卫生厅科技攻关计划项目(2008034):远志皂苷提取分离方法研究，负责人：王颖莉。