目的:研究超声波提取蜘蛛香中缬草素的最佳工艺.方法:采用4因素3水平L9(34)正交试验法,以缬草素的得率为考察指标,对超声波提取蜘蛛香中缬草素的工艺进行优化.在优选出的超声波最佳提取工艺条件下,将该法与渗漉法进行比较.结果:优选的超声波提取蜘蛛香中缬草素的最佳工艺条件为:药材粉碎,过40目筛,浸泡30min后,用相当于药材8倍重量的95%乙醇提取3次,每次提取40min.结论:超声波提取法可用于蜘蛛香中缬草素的提取.

This work aimed to optimize the extracting process of valtrate from Valeriana wallichii DC (V. jatamansi Jones) by supersonic wave. The orthogonal experiment L9（34） was adopted, and the gaining rate of valtrate was used as the evaluating criterion. The optimal conditions were as follows: the plant material was powdered, passed through a 40 mesh sieve, soaked for 30min, and extracted for 3 times with 8-fold weight 95% ethanol for 40min each time. The results showed that supersonic wave is suitable for the extraction of valtrate from V. wallichii.

国家自然科学基金项目(30572463):菌药蜘蛛香治疗肠激综合征(IBS)的物质基础及作用机理研究，负责人：闫兴丽。