目的:通过观察不同刺激方法对人体穴位一氧化氮(Nitric Oxide,NO)含量的影响,探讨不同治疗方法的作用机理.方法:对47例提供了知情同意书的健康志愿受试者进行实验.其中电针组11例,男性5例,女性6例;手针组13例,男性7例,女性6例;温灸组16例,男性8例,女性8例;非刺激对照组7例,男性4例,女性3例.年龄在25～45周岁.分别选取手厥阴心包经的间使穴和足太阳膀胱经的承山穴作为刺激穴位,并分别施以电针、手针、温灸刺激,对照组不给予刺激.分别在同侧经脉的郄门穴和承筋穴对穴位NO进行微透析采集并分析,比较不同刺激方法对NO含量的影响.结果:温灸组皮肤穴位NO含量高于电针组,电针组高于手针组,所有刺激组高于非刺激对照组(P＜0.05).结论:虽然不同的刺激方法对穴位NO含量的影响不同,但3种刺激方法均可使穴位NO含量显著升高.因此,穴位NO的表达可能是针灸作用的共同物质基础之一.

The mechanisms of different treatment methods were explored by determining the impact of different stimulation methods on the NO content at acupoints. The experiment was carried out for 47 healthy volunteers with informed consent. For the 11 cases in the electro-acupuncture group, 5 were male and 3 female. For the 13 cases in the acupuncture group, 7 were male, and 6 female. For the 16 cases in the warm-moxibustion group, 8 were male, and 8 female. For the 7 cases in the control group, 4 were male, and 3 female. The age was from 25 to 45 years old. Jianshi on the Pericardium Meridian of Hand Jueyin and Chengshan on the Bladder Meridian of Foot-Taiyang were selected as the stimulation points. Acupuncture, electro-acupuncture and warm-moxibustion were given separately. No stimulation was given in the control group. The NO content at Ximen and Chengjin was determined by the ipsilateral Meridians, and the effects of the different stimulation methods on the NO content were compared. The NO content at the acupoints was higher in the warm moxibustion group than in the electro-acupuncture group, higher in the electro-acupuncture group than in the acupuncture group, and higher in all three stimulation groups than in the control group（P <0.05）. The results showed that although the different methods had different effects on the NO content at the acupoints, they all significantly increased the NO content at the acupoints. The NO expression at acupoints may be one of the common material bases of acupuncture effects.

国家科学技术部等14部委和广东省人民政府共同主办的“2009’传统医药国际科技大会暨博览会”第五分会——“针灸理论和临床研究”论文，该论文为大会学术委员会评选的优秀论文；国家自然科学基金资助面上项目（30772830）：穴位从正常状态的“沉寂”到病理状态的“活化”过程和机制，负责人：荣培晶。