目的:借助前期研制成功的PMS肝气逆、郁两证大鼠模型检测其血清与不同脑区中E2、P、PRL含量,探索"肝失疏泄"的微观机制.方法:挑出动情周期非接受期大鼠40只,随机分为5组:正常对照组、PMS肝气逆证造模组、PMS肝气郁证造模组、PMS肝气逆证造模给药组、PMS肝气郁证造模给药组.肝气逆证大鼠模型以情志刺激为主多因素复合造模法制备,肝气郁证大鼠模型以慢性束缚应激法制备,模型复制成功后肝气逆大鼠模型用经前平颗粒干预,肝气郁证大鼠模型用经前舒颗粒干预.放射免疫法测定血清及中枢中E2、P、PRL含量,比较各组差异并进行分析.结果:旷场实验中,与正常对照组比较,大鼠造模后逆模组水平得分、垂直得分及可旷场实验总分均显著增加(P＜0.05),而郁模组大鼠则显著减少(P＜0.05),用药后这一趋势得到明显纠正(P＜0.05).肝气逆证模型大鼠症状呈现其血清中E2和P含量均呈下降趋势,肝气郁证模型大鼠血清中E2和P含量亦较正常对照组明显降低.中枢内激素变化与血清中相反.结论:PMS两证症状产生可能与体内性激素及其调节激素异常波动有关,经前平、经前舒颗粒可分别对外周血及中枢上述指标的异常变化起到调节作用.

PMS liver-qi invasion/depression rat models were established to detect the monoamine neurotransmitter content in sera and in different encephalic regions, thus exploring the possible mechanism of "liver losing regulation". 40 rats in the non-receptive phases were selected and randomly divided into 5 groups: the normal control group, the PMS liver-qi invasion group, the PMS liver-qi depression group, the PMS liver-qi invasion medication administration group, and the PMS liver-qi depression medication administration group. With the multiple factor combination method, emotion stimulation was given to prepare the PMS liver-qi invasion rat model, and chronic constraint stimulation was given to prepare the PMS liver-qi depression rat model. Then Jingqianping granules were administrated to the PMS liver-qi invasion rats, and Jingqianshu granules to the PMS liver-qi depression rats. The E2, P and PRL content in sera and in different encephalic regions were determined using the radioimmunity method. In the open-field experiment, the liver-qi invasion rats got much higher scores than the control rats, while the liver-qi depression rats got significantly decreased scores. This trend was retrieved after administration. Liver-qi invasion rats showed a descending tendency for the E2,P contents, and the liver-qi depression rats had a similar change in sera in the period of symptom presentation. A contradictory situation appeared in the center. These results indicated that PMS symptom emergence is likely related to the abnormal fluctuation in vivo of sex hormone and regulation hormone, and the two medicines aforementioned may cure the diseases by regulating the above abnormal changes.

国家科学技术部等14部委和广东省人民政府共同主办的“2009’传统医药国际科技大会暨博览会”第三分会——“传统医药基础理论研究”论文，该论文为大会学术委员会评选的优秀论文；科技部国家“十五”科技支撑计划课题（2006BAI08B05-10）：调肝方药干预经前期综合征肝气逆证的代谢机制，负责人：张惠云；国家重点基础研究发展计划（“973”计划）资助课题（2006CB504804）：愤怒和郁怒诱发情志病证发病机制及干预，负责人：乔明琦，张惠云；国家自然科学基金重点项目资助课题（30930110）：肝疏泄失常与调肝方药对不同脑区功能的影响及其机制研究，负责人：乔明琦；山东省科技厅科技攻关项目：情志病证非人灵长类动物模型研究平台构建，负责人：张惠云。