以病毒、细菌相关分子模式或具有不同证候上呼吸道感染患者的血清为激惹物,通过解表、清热类方剂的含药血清干预,观察哺乳动物巨噬细胞的Toll样受体(TLR)家族及其胞内信号转导通路接头蛋白的表达、信号诱导产生的细胞因子等的影响,探讨了方剂功能的分类学基础.结果发现,所试解表剂和清热剂作用于不同的TLR亚型;提示能识别病原体分子模式、启动先天性免疫、调节获得性免疫的TLR系统,可能是解表剂和清热剂功能分类的生物学基础之一;桂枝汤证患者血清导致的TLR表达异常,只有用桂枝汤可完全逆转其异常,也为"方证相应"提供初步的实验佐证.

This study was designed for isolating and identifying flavonoids from leaves of Cebera manghas. The chemical constituents were isolated using various column chromatography techiques, and their structures were elucidated based on their physicochemical constants and spectral data analysis. Eight flavonoids were obtained and determined to be quercetin (1), narigenin (2), aromadendrin (3), kaempferol-7-O-glucopyranoside (4), 5-O-glucopyranosyl-7, 4'-dihydroxy-3', 5'-dimethoxy-flavanone (5), kaempferol (6), kaempferol-3-O-rutinoside (7), and rutin (8). Among which, compounds 1-6 were isolated from genus Cerbera for the first time.

国家科学技术部等14部委和广东省人民政府共同主办的“2009’传统医药国际科技大会暨博览会”第三分会——“传统医药基础理论研究”论文，该论文为大会学术委员会评选的优秀论文；国家自然科学基金重大研究计划项目（90709016）：主治外感热病表证四方的“方证相应”实验研究，负责人：姜廷良。