目的：探讨与糖尿病肾病中医诊断相关的潜在生物标志物对于中医药现代化、客观化具有的重要意义。方法：应用高效液相色谱-三重四极杆质谱联用测定各证型DN患者和健康志愿者的血浆中8种硫醇氨基酸的含量，通过对照比较病例之间的差异。结果：DN患者体内Cys-gly和GSH的含量与健康人相比，显著降低；而SAH、SAM、Hcy、Cysta和Cys含量随着病情的加重有逐渐增高的趋势。根据SAH和Hcy能明显区分气阴两虚偏阴虚与阴阳两虚组。另外，气阴两虚偏气虚组患者体内的SAH含量与阴阳两虚组相比也有显著性差异。结论：体内Cys-gly、GSH含量可辅助DN的早期中医诊断，体内SAH和Hcy的含量与DN各证型具有一定的相关性。

Investigation of the potential biomarkers associated with the TCM diagnose of diabetic nephropathy is considered a contributing factor in facilitating TCM modernization and objectivity. This study carried out simultaneous quantitation of eight plasma aminothiols by HPLC-electrospray tandem mass spectrometry, so as to compare the difference between cases and the control. Compared with healthy people, the patients with diabetic nephropathy had dramatically degraded plasma GSH and Cys-gly concentrations, and their plasma SAH, SAM, Hcy, Cysta and Cys level gradually increased with the disease progress. SAH and Hcy indicated the difference between the Yin deficiency and Yin-Yang deficiency groups, especially when significant variance of the SAH concentration existed between Qi deficiency and Yin-yang deficiency. The results showed that plasma GSH and Cys-gly concentration may assist the early TCM diagnose, and the SAH and Hcy levels are associated with the syndrome type.

国家重点基础研究发展计划（“973”计划）项目(2005CB523503)：中医防治慢性肾功能衰竭临床方法的系统生物学研究，负责人：李平。