目的：观察局灶性脑缺血损伤后大鼠脑组织中Semaphorin 3A（Sema 3A）蛋白表达水平，以及通络救脑注射液对该因子表达的影响。方法：以线拴法制作大鼠大脑中动脉缺血模型（MCAO），设正常组、模型组及通络救脑注射液组，分别在造模后12h、24h、3d、7d，共4个时间点采取动物脑组织，测定脑梗死体积及脑组织中GAP43的表达水平，及Sema 3A的表达部位和蛋白表达水平。结果：通络救脑注射液能够减少脑缺血动物脑梗死体积，与模型组相比均有显著差异。结论：大鼠脑缺血损伤后Sema 3A表达增加与神经元的坏死有相关性，通络救脑注射液能够降低其表达水平，对缺血损伤的脑组织发挥保护作用。

This work observed the distribution of Semaphorin 3A(Sema 3A) in middle cerebral artery occlusion (MCAO) rats and the effect of Tongluo Jiunao injection (TLJN) on Sema 3A expression. Three groups were established, including the normal group, the control group, and the TLJN group. The expression of Sema 3A was observed by ELISA and double-immunofluorescent staining 12 h, 1 d, 3 d and 7 d after MCAO. The infarction volume was measured by TTC staining, and the level of GAP43 by ELISA. TLJN decreased the infarction volume and increased the expression of GAP43 compared with the control group. The expression of Sema 3A was increased after MCAO, and significantly decreased 3 d after TLJN injection. The results showed that the expression of Sema 3A may be related with the neuron injury after MCAO, and TLJN may protect the brain by inhibiting the expression of Sema 3A.

科学技术部“重大新药创制”科技重大专项（2009ZX09502-014）：基于脑病治疗的中药复方药理学及药效学评价关键技术研究，负责人：李澎涛；科学技术部国家“十一五”支撑计划（2006BAI08B05-04）：基于脑微血管内皮细胞功能物质发现的脑病药物筛选与效应分析新技术，负责人：潘彦舒；教育部科学技术研究重点项目（109023）从内皮-胶质细胞信息网络解析脑络功能及其病理基础，负责人：李卫红。