目的：分析肝络通对门静脉高压症大鼠肝组织中α7-烟碱样乙酰胆碱受体表达的影响特征，探讨肝络通治疗门静脉高压症的可能机制。方法：取体重240~260g的清洁级雄性SD大鼠40只，根据体重随机分为假手术组、2周模型组、2周肝络通组、4周模型组、4周肝络通组。模型组及给药组采用胆总管结扎术（CBDL）对大鼠进行造模，分别于造模2周、4周测量门静脉压力，并用免疫印迹法检测α7-烟碱样乙酰胆碱受体（nAchRα7）在肝组织中的表达。结果：胆总管结扎后2周和4周门静脉压力均显著升高，模型组nAchRα7的表达较假手术组明显增多，门静脉压力和nAchRα7的表达具有随术后时间延长而加重的一致性趋势。肝络通组门静脉压力显著低于模型组，术后4周作用最为显著；2周肝络通组nAchRα7的表达与2周模型组比较无显著差异，4周肝络通组较4周模型组显著减少。结论：肝组织nAchRα7的表达变化与门静脉压力变化具有一致性趋势，肝络通降低门静脉压力的作用可能与调节肝组织内nAchRα7的表达有关。

In this study, the expression of nAchRα7 in the liver tissue was observed after portal hypertension (PHT) was induced by common bile duct ligation. 40 male SD rats, weighing 240-260 g, were randomly divided into the sham group, the model group and the Glytan group according to the rat weight. Each group had 2 time points, i.e. 2 weeks and 4 weeks after PHT. PHT was induced by common bile duct ligation (CBDL) in rats. The portal pressure of each group was measured, and the expression of nAchRα7 in the liver was detected by Western blotting at each time point. The portal pressure and the expression of nAchRα7 of the model groups significantly increased 2 and 4 weeks after PHT. The portal pressure of the Glytan group decreased compared with the model group, especially 4 weeks after PHT. The expression of nAchRα7 in the liver of the Glytan group significantly decreased 4 weeks after PHT. Taken together, the portal pressure varied with the expression of nAchRα7 in the model group and the Glytan group. Glytan may down-regulate the portal pressure by affecting the nAchRα7 level in the liver.

科学技术部“重大新药创制”科技重大专项（2009ZX09102-144）：门脉高压症创新药物肝络通咀嚼片临床前研究，负责人：王新月。