本文着重介绍了脉冲场凝胶电泳（PFGE）在本草基因组测序中的应用，包括测定药用真菌染色体数目和基因组大小、构建基因组文库及绘制物理图谱，并对PFGE使用中一些待解决的问题做了简要介绍。

Pulsed Field Gel Electrophoresis(PFGE), which was developed in the 1980s, is mainly used to separate large linear DNA molecules (50 kb-12 Mb). With the development and implementation of the herbal genome program (HerbGP), PFGE is playing an increasingly critical role. In this study, we focus on the PFGE application in herbal genomic sequencing, including the measurement of the chromosome number and genome size of medicinal fungi, as well as construction of the genomic library and physical mapping. We also discuss the problems of how to use PFGE.

国家自然科学基金面上项目（30900113）：基于宏量ESTs的蛇足石杉转录组分析及石杉碱甲合成酶（HAS）基因的鉴定研究，负责人：罗红梅。