目的：通过建立脑缺血大鼠模型，观察开心解郁汤对双侧颈总动脉结扎大鼠行为学及脑血流量（CBF）的影响。方法：采用双侧颈总动脉永久结扎制备脑缺血动物模型；应用激光多普勒血流仪检测各组大鼠术后额叶、顶叶脑血流量;利用Morris水迷宫方法检测各组大鼠记忆功能。结果：与假手术对照组相比较，双侧颈总动脉永久结扎大鼠额叶、顶叶CBF明显降低，记忆功能显著下降。给予培元开郁中药3天后，与模型对照组比较，CBF加大，记忆功能障碍有所改善，但尚未达到统计学意义。结论：本实验通过建立脑缺血大鼠模型，证实了双侧颈总动脉结扎的适用性，同时说明开心解郁汤治疗双侧颈总动脉永久结扎大鼠的脑缺血有一定疗效，但尚需进行深入观察。

This work aimed to investigate the effect of Kaixin Jieyu Decoction on the behavior and cerebral blood flows of permanent bilateral carotid artery-ligated rats. The bilateral carotid artery-ligated rats were used as the animal models. By laser Doppler flowmetry detection, the rat frontal and parietal cerebral blood flow (CBF) were determined. The rat memory function was detected by the Morris water maze. Three days after administration of Kaixin Jieyu, the rat frontal and parietal CBF, as well as memory, of the experimental group were improved, but with no statistic significance. The results show that Kaixin Jieyu Decoction has some therapeutic effect on rat permanent cerebral ischemia caused by bilateral carotid artery ligation, but further investigation is needed.

国家自然科学基金（30672696）：益气开郁法对血管性抑郁症的干预与疗效机理研究，负责人：黄世敬；北京市自然科学基金（2093129）：慢性缺血脑白质损害凋亡基因活化及信号转导与中药干预，负责人：黄世敬。