目的：研究温和灸对亚急性衰老大鼠肝组织羰基毒化及p19ARF、p53 mRNA表达的影响。方法：SD大鼠随机分为正常组、模型组、艾灸治疗组和维生素E治疗组。连续大剂量腹腔注射D-半乳糖制备亚急性衰老大鼠模型，艾灸治疗组采用温和灸“肾俞”穴进行治疗。采用生物化学比色法检测各组大鼠肝组织蛋白质羰基含量，应用荧光定量PCR技术检测大鼠肝组织p19ARF、p53 mRNA的表达。结果：与正常组比较，模型组大鼠肝组织蛋白质羰基含量显著升高（P<0.01），p19ARF、p53 mRNA表达均显著升高（P<0.01）；与模型组比较，艾灸治疗组、维生素E治疗组肝组织蛋白质羰基含量显著下降(P<0.01，P<0.05)，p19ARF、p53 mRNA表达均显著降低（P<0.01，P<0.05）。结论：温和灸“肾俞”穴能降低亚急性衰老大鼠肝组织蛋白质羰基含量，下调肝组织p19ARF、p53 mRNA的表达。

This work aimed to study the effect of warming moxibustion on carbonyl stress and expression of p19ARF and p53 mRNA in the liver of subactue senile rats. SD rats were randomly divided into the normal group, model group, moxibustion treatment group and vitamin E treatment group. Subactue senile rats were prepared via continuous high-dose intraperitoneal injection of D-galactose. Moxibustion was performed at "Shenshu" points for the moxibustion treatment group. Protein carbonyl contents in livers were detected by biochemical chromatometry. p19ARF and p53 mRNA expressions in livers were detected by fluorescence quantitative PCR. Compared with normal rats, the model group showed significantly higher protein carbonyl contents, p19ARF and p53 mRNA expressions(P<0.01). Compared with the model group, the moxibustion treatment group and vitamin E treatment group showed significantly decreased protein carbonyl contents, p19ARF and p53 mRNA expressions(P<0.01, P<0.05). The results show that moxibustion at "Shenshu" points may reduce protein carbonyl contents, p19ARF and p53 mRNA expressions in the liver of subactue senile rats.

国家重点基础研究发展计划（“973”计划）（2009CB522900）：灸法作用的基本原理与应用规律研究，负责人：吴焕淦；国家自然科学基金项目（30701124）：艾灸延缓衰老大鼠组织羟基毒化反应的P19ARF/P53/P21CiPI信号调控机制，负责人：赵琛。