目的：研究杨树芽的化学成分，探明蜂胶和杨树芽内在成分差异。方法：采用溶剂提取和柱层析色谱分离，根据化合物理化性质和光谱数据鉴定其结构。结果：从加拿大杨树芽的正丁醇提取物和水提取物中分离得到3个苷类化合物和1个黄酮类化合物，分别为高良姜素（1）、乔松素-5-O-β-D-葡萄糖苷（2）、紫丁香苷（3）和水杨苷（4）。结论：化合物2和3为首次从杨属植物中分离得到，同时首次报道了化合物2的1H-NMR和13C-NMR数据。

Chemical components of the buds of P. canadensis were separated with various chromatographic techniques. The structures were elucidated by means of spectral analysis and physicochemical properties. Three glucosides and one flavonoid were isolated and identified as galangin (1), Verecundin (pinocembrin-5-glucoside, 2), syringin (3) and salicin (4). Compound 2 and 3 were isolated from Populus L. and reported for the first time. The 1H-NMR and 13C-NMR of compound 2 were reported for the first time.

科学技术部科研院所社会公益研究专项(2004DIB4J144)：中国蜂胶质量评价技术和标准研究，负责人：赵静。