目的：在前期研究基础上，以顶会穴、胃穴、火衰穴、肾穴为切入点，通过实验研究探讨蒙医灸疗法抗衰老组穴配伍规律。方法：将120只Wistar大鼠随机分二穴组和三穴组，采用D-半乳糖造模方法，观察和检测体重变化、SOD、CAT、MDA变化、脾脏（胸腺）指数以及学习能力、记忆力等指标。结果：从血清SOD、CAT、MDA变化、脾脏（胸腺）指数以及学习能力、记忆力等相关指标的观察发现，二穴组配伍中的顶会穴、火衰穴配伍组和三穴组配伍中的顶会穴、火衰穴、肾穴配伍组与模型组比较，SOD活性、MDA、CAT含量均无显著差异（P>0.05），其他配伍组SOD活性、CAT含量均明显提高（P<0.01）。从大鼠脾脏、胸腺指数变化上看，各配伍组大鼠脾脏、胸腺指数与模型对照组比较，明显提高（P<0.01或P<0.05），而与正常对照组比较无明显差异（P>0.05）。顶会穴与火衰穴配伍组、肾穴与火衰穴配伍组以及顶会穴、火衰穴、肾穴配伍组学习能力、记忆力与模型对照组比较无明显差异（P>0.05）。结论：①D-半乳糖衰老模型适用于蒙医灸疗法抗衰老有效穴位的筛选和配伍规律实验研究领域。②具有抗衰老作用的顶会穴、胃穴、火衰穴、肾穴在不同配伍组穴情况下显示出配伍禁忌。③该研究为灸疗法抗衰老临床提供了客观依据，能够促进蒙医灸疗法作用机理研究。

This study aimed to study Mongolian moxibustion rules for anti-aging through experiments. Based on previous studies, acupoints such as Dinghui, Stomach point, Huoshuai, and Kidney point are used in the study. All 120 Wistar rats were divided into the two-acupoint group and three-acupoint group randomly. D-galactose was used in the establishment of models. The change of indexes, such as weight, SOD, CAT, MDA was observed. The spleen (thymus) index, study ability and memory were also measured. The results showed that there was no obvious difference of the content change of SOD, MDA and CAT between the two-acupoint (Dinghui and Huoshuai) group and the three-acupoint (Dinghui, Huoshuai and Kidney point) group (P>0.05). The SOD was also obviously increased in groups with other combinations (P<0.01). Compared with the model group, the spleen (thymus) index was obviously increased in all groups (P<0.01, or P<0.05). But there was no remarkable difference (P>0.05) comparing with normal control group. There was no obvious difference among the model group with Dinghui-Huoshuai combination, Kidney-Huoshuai combination, and Dinghui-Huoshuai-Kidney combination on the aspect of study ability and memory (P>0.05). The results showed that D-galactose aging model was suitable for the research of screening effective acupoints and combination rules for applying Mongolian moxibustion for anti-aging treatment. The combination contradictions were shown among different combinations with Dinghui, Stomach point, Huoshuai, and Kidney point. This study also provided objective basis for Mongolian moxibustion in anti-aging treatment. It also provided experiences for the mechanism of Mongolian moxibustion.

国家自然基金地区科学基金项目（30760323）：蒙医灸疗法抗衰老组穴配伍规律实验研究，负责人：斯钦图。