目的：建立中医阴黄证动物模型，系统评价茵陈四逆汤对大黄、乙醇、琢-异硫氰酸萘酯（ANIT）三因素复合诱导的大鼠阴黄证的治疗作用。方法:70 只Wistar 大鼠随机分为7 组，分别为空白组、模型组、模型恢复组、茵陈四逆汤低剂量组、茵陈四逆汤中剂量组、茵陈四逆汤高剂量组、阳性药对照组。观察大鼠一般生物学状态，检测谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）、酌-L-谷氨酰转肽酶（酌-GT）、总胆红素（TB）、直接胆红素（DB）、总胆汁酸（TAB）、血清白介素2（IL-2）的活性和效价以及环磷酸腺苷与环磷酸鸟苷比值（cAMP/cGMP），同时观察大鼠肝脏组织病理学情况。结果：阴黄证大鼠出现明显阳虚表现。模型组、模型恢复组ALT、AST、ALP、酌-GT、TB、DB、TAB、IL-2 活性显著高于正常对照组（P<0.01），治疗组上述指标，与模型组及模型恢复组比下降显著（P<0.01）；模型组、模型恢复组cAMP/cGMP 比值显著低于正常对照组（P<0.01），治疗组cAMP/cGMP 比值与模型组及模型恢复组比升高显著（P<0.01）。同时，组织病理学也得出上述相同的结论。结论:茵陈四逆汤对阴黄证大鼠具有良好的治疗作用。

This work aimed to investigate the effect of Yinchensini-Tang on Yin jaundice rats. 70 rats were randomly divided into 7 groups: the control group, the model group, the model recovery group, the Yinchensini-Tang low-dose group, the Yinchensini-Tang middle-dose group, the Yinchensini-Tang high-dose group, and the UDCA group. The changes of liver function and hepatic pathology and behavior were observed. The ALT, AST, ALP, r-GT, TB, DB, TAB and IL-2 in the model group and model recovery group were significantly higher than in the control group (P<0.01). The ALT, AST, ALP, r-GT, TB, DB, TAB and IL-2 in the Yinchensini-Tang high-dose group were significantly lower than in the model group and model recovery group (P<0.01), The cAMP/cGMP in the model group and model recovery group was significantly lower than in the control group (P<0.01). The cAMP/cGMP in the Yinchensini-Tang high-dose group was significantly higher than in the model group and model recovery group (P<0.01). The results showed that high-dosed Yinchensini-Tang could amend the changes of hepatic pathology in Yin jaundice rats. Thus Yinchensini-Tang has better effect on Yin jaundice rats.

国家重点基础研究发展规划（“973”）项目（2005CB523406）：基于体内直接作用物质的类方配伍规律研究，负责人：王喜军；国家自然科学基金重大研究计划（90709019）：基于代谢物组学的中医证候本质及方证相应的研究，负责人：王喜军。