目的：优化桦褐孔菌多糖的提取工艺。方法：采用均匀设计分别对影响提取和醇沉的因素进行优化。结果：最佳提取工艺为固液比1颐15，提取时间90min，提取次数2 次。最佳醇沉工艺为生药浓度1.0g·mL-1，乙醇终浓度65%。结论：优化的桦褐孔菌多糖提取工艺和醇沉工艺经济、稳定、合理可行。

This study aimed to optimize the extraction techniques of polysaccharide from Inonotus obliquus by uniform design. The content of I. obliquus polysaccharides was determined by spectrometry method, which takes anhydrous glucose as standard and 5% phenol and sulfuric acid as color-developing agent, in order to investigate the extraction and alcohol-purification technologies of I. obliquus polysaccharides. The extraction and alcohol-purification technologies of I. obliquus polysaccharides were optimized by employing the uniform design method. Results showed that the best extraction technology was when the I. obliquus and water ratio is 1:15. And the extraction should be given twice and 90 minutes for each time. The best alcohol-purification technology was to add alcohol into the concentration of 1.0 g/mL crude herb to 65% with vigorously rabbling. The conclusion is that the extraction and alcohol-purification technologies of I. obliquus polysaccharides optimized by uniform design method are economical, stable and efficient.

黑龙江省教育厅科技攻关项目（11513103）：桦褐孔菌降糖作用药效物质基础及其药效学的研究，负责人：孙海峰。