目的：建立测定大鼠体内血浆和唾液中盐酸川芎嗪含量的HPLC分析方法。方法：采用色谱柱：Diamonsil（R）C18 柱（250mm×4.6mm，5μm）；流动相∶甲醇∶水（70∶30，v∶v）；柱温：30℃；流速：0.8mL·min-1； 检测波长：295nm。结果：血浆和唾液中盐酸川芎嗪浓度在0.5～50μg·mL-1和0.3～30μg·mL-1范围内相关系数分别为0.9992和0.9999，线性关系良好；盐酸川芎嗪血浆和唾液的相对回收率分别为97.03%～101.19%和99.55%～99.88%；日内、日间精密度的RSD<10%。结论：建立了盐酸川芎嗪唾液样品检测方法以及用HPLC法同时测定大鼠体内血浆和唾液中的含量方法，该方法简便、快速、准确，为盐酸川芎嗪的临床药动学研究提供了新的方法学基础。

This study aimed to establish HPLC determination for TMPH concentration in plasma and saliva of rats. The HPLC was performed on a Diamonsil(R) C18 column (250mm×4.6mm, 5μm) using a mobile phase consisting of methanol-water (70:30, v:v) at 30℃ with the flow rate of 0.8mL· min-1. And the detection wavelength is at 295nm. The result showed that the calibration curve was linear in the range of 0.5- 50.0μg·mL-1 (r=0.9992) in plasma and 0.3-30.0μg·mL-1 (r=0.9999) in saliva for the determination of TMPH. The average recoveries of TMPH in plasma and saliva were 97.03%-101.19% and 99.55%-99.88%, respectively. The intra- and inter-day RSDs were less than 10%. It is concluded that this method is simple, rapid, and accurate. It provides a new methodological basis for the clinical pharmacokinetics study of TMPH.

黑龙江省哈尔滨市留学回国基金资助项目（2005AFLXJ020）：中药中生物碱在唾液中分布的研究，负责人：李秋红。