近年来,中药DNA条形码分子鉴定得到快速发展,加快了中药鉴定标准化的进程。通过中药DNA条形码鉴定研究,我国首次提出将ITS2序列作为药用植物鉴定的通用条形码序列,并建立以ITS2为核心、psbA-trnH为补充序列的植物类药材DNA条形码鉴定体系和以COI序列为核心、ITS2为辅助序列的动物类药材DNA条形码鉴定体系,为中药DNA条形码鉴定的发展奠定了坚实基础。本文结合课题组研究工作就构建中药DNA条形码鉴定体系进行了综述,包括中药DNA条形码序列的筛选确定、中药DNA条形码鉴定流程和中药DNA条形码鉴定数据库系统构建,并介绍了中药DNA条形码鉴定的研究方向。

DNA barcoding in Traditional Chinese Materia Medica has developed rapidly in recent years,which accelerates the progress of standardization in identifying Traditional Chinese Materia Medica.Chen et al.first proposed that ITS2 locus can be used as the standard barcode for identifying medicinal plant species based on an extensive study on DNA barcoding in Traditional Chinese Materia Medica.Meanwhile,the systems for barcoding medicinal plants based on a two-locus combination of ITS2+psbA-trnH and for authenticating medicinal animals based on COI+ITS2 have been established,providing a basis for the development of DNA barcoding in Traditional Chinese Materia Medica.In this review,we summarize the establishment of the system for barcoding Traditional Chinese Materia Medica,including the selection and determination of DNA barcodes,the identification process of DNA barcoding in Traditional Chinese Materia Medica and building the database system of DNA barcoding in Traditional Chinese Materia Medica.Moreover,we describe the perspective for further research on DNA barcoding in Traditional Chinese Materia Medica.

科学技术部卫生行业科研专项(200802043):药用植物DNA barcoding(条形码)鉴定研究,负责人:陈士林