目的:建立检测止痢散等7种中兽药散剂中违规添加喹乙醇、乙酰甲喹的HPLC-PDAD法,并用MS进行确证。方法:根据处方和临床治疗量配制阴性中兽药散剂和阳性供试品;用90%乙腈提取,HPLC-PDAD法进行检测,并用MS进行确证。结果:检出限为0.05 g.kg-1,喹乙醇和乙酰甲喹在止痢散中的平均回收率分别为93.69%和101.22%。结论:该方法快速、简便、准确,可用于中兽药散剂中违规添加喹乙醇、乙酰甲喹的检测。

The study was aimed to establish a HPLC-PDAD method for determination of olaquindox and mequindox illegally adulterated into seven Chinese materia medica powders for animals.Powders and samples were prepared on the basis of prescriptions and dosage of the Chinese Veterinary Pharmacopoeia.Samples were extracted with 90% acetonitrile,determined by HPLC-PDAD,and then identified by MS.The result showed that the limit of detection of olaquindox and mequindox was 0.05 g/kg.The mean recovery of olaquindox in the Zhili powder was 93.7%.The mean recovery of mequindox in the Zhili powder was 101.2%.It concluded that the method is rapid,simple and accurate.It can be used in the determination of olaquindox and mequindox illegally adulterated into the Chinese materia medica powders for animals.

中国兽医药品监察所所级科研项目:常用中兽药违规添加西药的检测研究,负责:高光